Human HepG2 Hepatocellular Carcinoma Cells

Human HepG2 Hepatocellular Carcinoma Cells


Cryopreserved vial of HepG2 hepatocellular carcinoma cells (~500,000 cells)

SKU: CR1015-500 Category:

Product Overview

HepG2 is a human cell line isolated from a hepatocellular carcinoma of 14 year old male donor. HepG2 cells are used in a wide range of studies, from the oncogenesis to the cytoxicity of substances on the liver

These cells can be used for (i) the study of carcinogenesis as a surrogate for liver toxicity, (ii) the analysis of signaling events, (iii) molecular biology and (iv) protein-based assays. These immortalized hepatic cell lines can be used in place of biopsies for research purposes.

These HepG2 cells are homogenous and grow robustly in cell culture. Vial contains approximately 500,000 cells. Shipped with dry ice.

Cell Characteristics

Growth PropertiesAdherent
Donor Age14 years old
KaryotypeModal number = 55 (range = 50 to 60); has a rearranged chromosome 1
TumorigenicNo; No, in immunosuppressed mice Yes, in semisolid medium
Genes expressedalpha-fetoprotein (AFP, alpha-fetoprotein); albumin; alpha2 macroglobulin (alpha-2-macroglobulin); alpha1 antitrypsin (alpha-1-antitrypsin); transferrin; alpha1 antichymotrypsin (alpha-1-antichymotrypsin); haptoglobin; ceruloplasmin; plasminogen, complement (C4); C3 activator; fibrinogen; alpha1 acid glycoprotein (alpha-1 acid glycoprotein); alpha2 HS glycoprotein (alpha-2-HS-glycoprotein); beta lipoprotein (beta-lipoprotein); retinol binding protein (retinol-binding protein)
Expression markersInsulin: insulin-like growth factor II (IGF II)

Stability & Storage

 Storage Time
Human HepG2 Hepatocellular Cells
-80°C (preferably in the vapor phase of a liquid nitrogen storage unit)
12 months
Human HepG2 Hepatocellular Carcinoma Expansion Media (not included)
3 months
Complete Media (see Media Formulation Instructions)
Not applicable

Avoid repeated freeze-thaw cycles for cells. Avoid repeated exposure to room temperature and light for medias.


Thrombospondin 2 is a Key Determinant of Fibrogenesis in Non-Alcoholic Fatty Liver Disease
Kimura, T et al. | Liver International 2024 FEB.

Comparison of Amine-Modified Polymeric Stationary Phases for Polar Metabolomic Analysis Based on Unified-Hydrophilic Interaction/Anion Exchange Liquid Chromatography/High-Resolution Mass Spectrometry (Unified-HILIC/AEX/HRMS)
Ikeda, K et al. | Mass Spectrometry 2024 JAN.

Hydroxyapatite Nanocoating on Calcium Peroxide Microparticles for Sustained Oxygen Release
Tomioka, D et al. | Chemistry of Materials 2023

Decellularized liver hydrogel enhances cell engraftment in orthotopic hepatocyte transplantation by promoting cell-cell interaction and angiogenesis.
Udagawa, D et al. | Research Square 2023 MAY.

Relationship between clock gene expression and CYP2C19 and CYP3A4 with benzodiazepines.
Tani, N et al. | Human & Experimental Toxicology 2023 APR.

Effect of methamphetamine on clock genes and drug-metabolizing enzyme expression.
Tani, N et al. | Human & Experimental Toxicology 2022 AUG.

Protocols & Documentation

Download: Product Information Sheet